Composite

Part:BBa_K3001003:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-16)


Cas13a Lbu + T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 107
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 297
    Illegal BglII site found at 845
    Illegal BglII site found at 1781
    Illegal BglII site found at 1991
    Illegal BglII site found at 2045
    Illegal BglII site found at 2276
    Illegal BglII site found at 2549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
    Illegal NgoMIV site found at 2497
    Illegal NgoMIV site found at 3133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs to be overexpressed in an E. coli strain with rare codons.


Source

Ordered from Addgene

References